FAN-C is a Python (3.6+) toolkit for the analysis and visualisation of Hi-C data.
For common tasks, you can use the
fanc executable. For more advanced analyses, FAN-C
can be imported as a powerful Python module.
Before installing FAN-C, make sure you have all the prerequisites installed on your system. Specifically, FAN-C uses the HDF5 file format internally (via PyTables) to handle the large amount of data typically generated in Hi-C experiments. The minimum required version of HDF5 is 1.8.4, which may already be installed on your machine.
It is generally not necessary to install HDF5 manually, as some version will typically be
installed on any current Unix system, including macOS. If you can install FAN-C via pip
(see below) and
fanc --version returns a version number, you are most likely good to go.
If you are on Linux, download the source code of the latest version from the HDF5 website and unpack it.
# make a new directory mkdir hdf5-build cd hdf5-build # replace xx with current version number wget https://support.hdfgroup.org/ftp/HDF5/current/src/hdf5-1.8.xx.tar.gz # unpack tar xzf hdf5-1.8.xx.tar.gz cd hdf5-1.8.xx/ # use --prefix to set the folder in which HDF5 should be installed # alternatively, you can omit --prefix=... here and run # sudo make install to install globally (requires admin rights) ./configure --prefix=/path/to/hdf5/dir make make install
If you are on OS X or macOS, we highly recommend using the fantastic Homebrew. Then you can simply:
brew install firstname.lastname@example.org
To ensure that PyTables, the Python library that uses HDF5, finds the correct HDF5 version, we need to set an environment variable pointing to the installation directory:
# on Linux, this is the same /path/to/hdf5/dir that you used above # on macOS, this is wherever brew installs its recipies (typically /usr/local/Cellar) # - you can find this by running 'brew info hdf5' export HDF5_DIR=/path/to/hdf5/dir
The simplest way to install FAN-C is via pip:
pip install fanc
and that should be all you need! If you are not the owner of the Python installation, try:
pip install --user fanc
You can also directly download the FAN-C source code from Github by cloning its repository. The installation is then done via setup.py:
git clone http://www.github.com/vaquerizaslab/fanc cd fanc pip install .
FAN-C can now be accessed via command line (
fanc for analysis,
fancplot for plotting)
or as a Python 3.6+ module (
If you want the latest improvements, and can live with a higher likelihood of unresolved issues,
you can become a beta tester and check out the
dev channel! We would appreciate feedback on
GitHub for any issue that you encounter.
git clone http://www.github.com/vaquerizaslab/fanc git checkout dev cd fanc pip install -e .
We recommend working from a clean conda environment using Python 3.7:
conda create -n my_fanc_env python=3.7 conda activate my_fanc_env conda install fanc
On some systems the installation of FAN-C using the above method will fail with a Cython dependency error. In this case, installing Cython and pysam prior to FAN-C might solve the issue:
pip uninstall fanc pip uninstall pysam pip install Cython pip install pysam pip install fanc
If you are still experiencing problems during the installation, please raise an issue on GitHub.
If you want to build this documentation on your local machine, first make sure to install the prerequisites by running
pip install sphinx sphinx_rtd_theme sphinx-argparse
Then navigate to the
docsrc folder (assuming you are in the
fanc base folder):
make to get a list of possible documentation outputs, for HTML use:
You will find the html output in
For this example, we are going to use the command
fanc auto (see Generating Hi-C matrices with fanc) to
construct a Hi-C map from a subset of a previously published adrenal tissue data set
(SRR4271982 of GSM2322539).
You can download the files from our Keeper library:
wget -O examples.zip "https://keeper.mpdl.mpg.de/d/147906745b634c779ed3/files/?p=/examples.zip&dl=1" unzip examples.zip cd examples
From the examples folder, run:
fanc auto SRR4271982_chr18_19_1.fastq.gzip SRR4271982_chr18_19_2.fastq.gzip output/ \ -g hg19_chr18_19.fa -i hg19_chr18_19/hg19_chr18_19 -n fanc_example -t 4 -r HindIII \ --split-ligation-junction -q 30 --run-with test
--run-with test argument causes
fanc to only print the commands it would execute, but
to exit before running any processing steps. Use this to review the pipeline and ensure you chose
the right parameters and that there are no errors.
When you remove the
--run-with test argument,
fanc will work through the pipeline.
On a modern desktop computer with at least four computing cores the command should take less
than 30 minutes to finish. It will generate several binned, bias-corrected Hi-C matrices from the
You can read details about
fanc auto and all of its parameters in Generating Hi-C matrices with fanc.
We can plot the newly generated Hi-C maps easily using the
fancplot command. Simply execute
fancplot chr18:63mb-70mb -p triangular -vmax 0.05 output/hic/binned/fanc_example_100kb.hic
This will plot the region 63-70Mb of chromosome 18 in the familiar Hi-C plot. Note that this dataset is very small and hence the quality of the matrix not particularly great - but TADs are clearly visible.
You can find details about the plotting executable
fancplot in Basic usage.
Find out more about
fanc auto and its parameters in Generating Hi-C matrices with fanc. If you are interested
in customising individual steps of the pipeline, or in exploring all of FAN-C’s analysis options,
have a look at Individual pipeline steps. For more plotting functions, continue to Basic usage.
To access FAN-C functionality from within Python, check out fanc Python module.